{"id":674,"date":"2022-10-12T16:16:48","date_gmt":"2022-10-12T15:16:48","guid":{"rendered":"https:\/\/research.reading.ac.uk\/chemical-analysis-facility\/?p=674"},"modified":"2025-11-20T17:21:05","modified_gmt":"2025-11-20T17:21:05","slug":"bruker-hd-400-mhz","status":"publish","type":"post","link":"https:\/\/research.reading.ac.uk\/chemical-analysis-facility\/bruker-hd-400-mhz\/","title":{"rendered":"Bruker HD 400 MHz"},"content":{"rendered":"

[vc_row][vc_column width=”1\/3″][vc_single_image image=”560″ img_size=”full”][\/vc_column][vc_column width=”2\/3″][vc_column_text]The HD400 is a two-channel NMR instrument running ICON NMR 4.1 under TOPSPIN 3.6. It incorporates a 9.39 T magnet without active shielding and is equipped with a 5mm z-gradient direct observation (1<\/sup>H\/13<\/sup>C\/19<\/sup>F)- BBFO probe and a 60-position BACS sample changer.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Find out more<\/h3>\n

[\/vc_column_text][vc_tta_accordion active_section=”1″ collapsible_all=”true”][vc_tta_section title=”When do I use the Bruker DPX 400 MHz?” tab_id=”1668506018299-f599c205-93c4″][vc_column_text]Of the two Open Access NMR instruments, the HD400 is superior for performing heteronuclear direct detection as the probe for this instrument has optimised sensitivity for heteronuclei. Therefore, if you have a limited amount of sample (see Sample Requirements for Experiments in NMR for the minimum amount of sample which is required in order for each of the above experiments to succeed) you will get much better results from running your 1D-NMR 13<\/sup>C, DEPTQ and DEPT-135 experiments on the HD400, in preference to the Nano400. Conversely, 2D-NMR experiments involving direct observation of proton, such as COSY, NOESY, edited-HSQC, and HMBC will give better results on the Nano400, if only a small amount of sample is available. Very small amounts of sample, which cannot be run in a reasonable time on either of the Open Access NMR instruments, can be submitted to the Internal NMR Service (either the Bruker Avance III 500 MHz or the AV700 NMR instruments) for acquisition by the NMR Staff.<\/p>\n

 <\/p>\n

By default, six ICONNMR Experiments: 1H (1D-), 13C (1D-), DEPT-135 (1D-), Edited-HSQC (2D-), HMBC (2D-) and COSY (2D-) are made available to all new users of the HD400. However, the HD400 can also run direct-detection experiments from many other nuclei, such as 31P, 19F and 15N. If you require these or other experiments, then please consult with the NMR Staff who will add them to your user account.[\/vc_column_text][\/vc_tta_section][vc_tta_section title=”What experiments are available?” tab_id=”1668506018261-e2868634-21bd”][vc_column_text]By default, six ICONNMR Experiments:\u00a01<\/sup>H (1D-),\u00a013<\/sup>C (1D-), DEPT-135 (1D-), Edited-HSQC (2D-), HMBC (2D-) and COSY (2D-) are made available to all new users of the DPX400. However, the DPX400 can also run direct-detection experiments from many other nuclei, such as\u00a031<\/sup>P,\u00a019<\/sup>F and\u00a015<\/sup>N. If you require these or other experiments, then please consult with the NMR Staff who will add them to your user account.[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/3″][vc_column_text]<\/p>\n

Instrument availability<\/h3>\n

[\/vc_column_text][vc_column_text]The DPX400 is available only to research workers from the University of Reading.[\/vc_column_text][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n
Monday<\/td>\n00:00\u201309:00
\n12:00\u201300:00<\/td>\n<\/tr>\n
Tuesday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n
Wednesday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n
Thursday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n
Friday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n
Saturday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n
Sunday<\/td>\n00:00\u201300:00<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]<\/p>\n

Useful information<\/h3>\n

[\/vc_column_text][vc_column_text]All users must have received training in use of the open access NMR instruments (provided by the NMR Staff).<\/p>\n

When using the Open Access NMR instruments you are expected to perform NMR Sample Preparation yourself.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]<\/p>\n

\n
\n

Contact<\/strong><\/h3>\n

For more information about the instrument and its availability contact:<\/span><\/p>\n<\/div>\n<\/div>\n

\n
\n

caf.nmr@reading.ac.uk<\/u><\/a><\/p>\n<\/div>\n<\/div>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column width=”1\/3″][vc_single_image image=”560″ img_size=”full”][\/vc_column][vc_column width=”2\/3″][vc_column_text]The HD400 is a two-channel NMR instrument running ICON NMR 4.1 under TOPSPIN 3.6. It incorporates a 9.39 T magnet without active shielding and is equipped…Read More ><\/a><\/p>\n","protected":false},"author":219,"featured_media":560,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"__cvm_playback_settings":[],"__cvm_video_id":"","footnotes":""},"categories":[25,17],"tags":[],"coauthors":[15],"class_list":["post-674","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bruker-dpx-400-mhz","category-nmr"],"acf":[],"yoast_head":"\nBruker HD 400 MHz - Chemical Analysis Facility (CAF)<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/research.reading.ac.uk\/chemical-analysis-facility\/bruker-hd-400-mhz\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Bruker HD 400 MHz - Chemical Analysis Facility (CAF)\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=”1\/3″][vc_single_image image=”560″ img_size=”full”][\/vc_column][vc_column width=”2\/3″][vc_column_text]The HD400 is a two-channel NMR instrument running ICON NMR 4.1 under TOPSPIN 3.6. 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