{"id":464,"date":"2017-09-26T10:25:12","date_gmt":"2017-09-26T09:25:12","guid":{"rendered":"http:\/\/ironmicrobiome.com\/?p=464"},"modified":"2019-10-01T17:03:49","modified_gmt":"2019-10-01T16:03:49","slug":"exploring-human-host-microbiome-interactions-in-health-and-disease","status":"publish","type":"post","link":"https:\/\/research.reading.ac.uk\/iron-and-microbiome\/exploring-human-host-microbiome-interactions-in-health-and-disease\/","title":{"rendered":"Iron and haem contained in the gut model medium caused significant changes in the microbiota composition"},"content":{"rendered":"


\n\"\"<\/a><\/span>Our recent work \u00a0has been aimed to evaluate \u00a0how the iron sources (18 mM FeSO4 and Hemin 77 mM) included in a Gut model medium (GMM) \u00a0influence the metabolic activity and composition of the gut microbiota in vitro<\/em>. The presence of FeSO4 and Hemin\u00a0caused significant changes in the microbiota composition that could have detrimental effects on health. Iron\/haem supplements reduced the alpha diversity in the microbiota and increased the proportion of Enterobacteriales and Bacteroidales (with a large number of potential pathogens within these groups). Conversely, in absence of iron\/haem, the reduction of the potential pathogen C. perfringens<\/em> and the increases of some butyrate producers (Lachnospira<\/em> spp. Roseburia<\/em> spp and Eubacterium<\/em> spp.) and B. animalis<\/em> could indicate potential health benefits in the colon environment. These changes seemed to be insufficient to affect SCFA levels, although higher ammonia levels were observed upon Fe supplementation in 2 out of 3 donors.<\/span><\/p>\n

In addition, we have been working in the optimisation of the in vitro<\/em> models that will be employed in the project to study the influence of iron and other dietary components on the\u00a0microbiota. For instance, even without Fe\/haem supplementation, GMM contains high Fe levels (25 mM), with Mucin being the principal contributor. A modified GMM has now been defined, that has far lower Fe levels (<3 mM) and specified levels of vitamins and essential\/trace elements. This medium is being validated for use in our future work.<\/span><\/p>\n

These results have been presented in the 6th Exploring Human Host-Microbiome Interactions in Health and Disease<\/em> \u00a0organized by Wellcome Genome Campus and held during Sep 13 – 15, 2017 in Hinxton, Cambridge.\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Our recent work \u00a0has been aimed to evaluate \u00a0how the iron sources (18 mM FeSO4 and Hemin 77 mM) included in a Gut model medium (GMM) \u00a0influence the metabolic activity…Read More ><\/a><\/p>\n","protected":false},"author":219,"featured_media":590,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"__cvm_playback_settings":[],"__cvm_video_id":"","footnotes":""},"categories":[8],"tags":[],"class_list":["post-464","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"aioseo_notices":[],"yoast_head":"\nIron and haem contained in the gut model medium caused significant changes in the microbiota composition - 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