{"id":20637,"date":"2020-06-23T10:52:38","date_gmt":"2020-06-23T09:52:38","guid":{"rendered":"https:\/\/research.reading.ac.uk\/research-blog\/?p=20637"},"modified":"2021-11-15T10:52:38","modified_gmt":"2021-11-15T10:52:38","slug":"new-low-cost-technologies-to-tackle-global-challenges-early-career-researchers-at-reading-are-helping-to-shapetheworld","status":"publish","type":"post","link":"https:\/\/research.reading.ac.uk\/research-blog\/2020\/06\/23\/new-low-cost-technologies-to-tackle-global-challenges-early-career-researchers-at-reading-are-helping-to-shapetheworld\/","title":{"rendered":"New, low-cost technologies to tackle global challenges – Early Career Researchers at Reading are helping to #shapetheworld"},"content":{"rendered":"

Dr Sarah Needs and Dr Sophie Jegouic, biomedical engineers at Reading, have designed and built a low-cost robot that takes high resolution images of experiments to speed up tests for antibiotic resistance and COVID-19 antibodies. Here, for #INWED20, they explain how they are using open source hardware, 3D printing and a raseberry pi computer to address these global challenges.<\/strong><\/span><\/p>\n

<\/h3>\n
\"\"<\/a>
Sophie Jegouic<\/figcaption><\/figure>\n
\"\"<\/a>
Sarah Needs<\/figcaption><\/figure>\n

Biomedical Technology Lab at Reading<\/h3>\n

<\/p>\n

Our Biomedical Technology Lab builds simple low-cost solutions for current and future healthcare problems. We are a team from a biological sciences background and are embracing interdisciplinary working, developing new technologies to tackle global challenges ranging from antimicrobial resistance to Covid-19 testing.<\/p>\n

Rapid prototyping is the process we use to quickly make physical models using computer-aided designs. We mainly use 3D printing and low-cost electronics to make and test imaging systems designed to be compatible with the bioassays we are developing. Commercial printers are increasingly affordable and allow the design of a new piece of equipment and to print it within a day.<\/p>\n

Antimicrobial resistance tests<\/h3>\n

Antimicrobial resistance is a global challenge and threatens the effective treatment of infections caused by bacteria. I (Dr Sarah Needs) am a postdoctoral researcher in Dr Al Edwards lab in the School of Pharmacy, and am using these techniques to develop rapid antimicrobial resistance tests.<\/p>\n

Traditional microbiology techniques can take 48 hours to determine antibiotic resistance. However, using micro-devices and following a colour change which corresponds to bacterial growth, this can be identified within hours. To help in this study we developed the POLIR, (Raspberry Pi camera Open-Source Laboratory Imaging Robot). This system was developed based on designs for 3D printers with the addition of a raspberry pi camera.<\/a><\/p>\n

\"3D<\/a>
POLIR. Image taken by Dr Sophie Jegouic<\/figcaption><\/figure>\n

Using this design, the raspberry pi camera can move around the imaging area and take pictures of the experiment at different locations and times, allowing high resolution time-lapse imaging. This allows over 2000 individual tests to be screened in a single experiment. In June 2019 we successfully installed a POLIR in Universiti Teknologi Malaysia for use with microbiology tests.<\/p>\n

 <\/p>\n

\"\"<\/a>
Sarah Needs assembling POLIR in Malaysia. Image taken by Dr Al Edwards<\/figcaption><\/figure>\n

Using the robot in our lab, we have decreased the amount of time spent in-lab for researchers, while increasing the quality and quantity of so-called kinetic data. Our hope is that other laboratories can implement versions of this open hardware solution to reduce the amount of time spent imaging in the lab by researchers.<\/p>\n

Covid-19 Antibody Tests<\/h3>\n

Dr Sophie Jegouic, a postdoctoral researcher in biomedical sciences, has been using similar techniques using raspberry pi cameras and 3D printed components in the development of rapid COVID-19 antibody tests. Currently, most Covid-19 tests detect viral RNA using a technique called PCR, and can only identify if someone has an active infection. Antibody tests allow the detection of Covid-19 antibodies in the blood which can identify if someone has had an infection in the past.<\/p>\n

There are two types of tests available; laboratory tests, which tend to be accurate but require a significant amount of equipment and user-training or rapid tests that are cheaper and can be performed outside of a lab, but tend to be less accurate.<\/p>\n

\"\"<\/a>
Development of Covid-19 antibody tests. Image taken by Dr Sophie Jegouic<\/figcaption><\/figure>\n

We have been working on tests that are more portable than laboratory tests but still as accurate. The test involves coating micro-devices with a viral protein. This protein alone is not infectious, but allows antibodies in a blood sample to bind to it. The amount of antibody binding can then be measured by the fluorescent output which is linked to the amount of antibody present.<\/p>\n

While the laboratory experimental setups measuring these types of tests require expensive and bulky laboratory equipment, we use an imaging setup designed in-house and 3D printed. This can also be used with a smartphone camera, potentially allowing this test to be done outside of a lab.<\/p>\n

Rapid prototyping means we can develop new bioassays quickly, responding to real world challenges.<\/p>\n","protected":false},"excerpt":{"rendered":"

Dr Sarah Needs and Dr Sophie Jegouic, biomedical engineers at Reading, have designed and built a low-cost robot that takes high resolution images of experiments to speed up tests for…Read More ><\/a><\/p>\n","protected":false},"author":128,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"__cvm_playback_settings":[],"__cvm_video_id":"","footnotes":""},"categories":[1785],"tags":[1701,992,1122,1702,638,1700],"class_list":["post-20637","post","type-post","status-publish","format-standard","hentry","category-agriculture-food-and-health","tag-3d-printing","tag-antimicrobial-resistance","tag-biomedical-engineering","tag-covid-19-antibody-testing","tag-open-research","tag-polir"],"acf":[],"yoast_head":"\nNew, low-cost technologies to tackle 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