Our Publications

2024

• Sarıyer, R.M.; Gill, K.K.; Needs, S. H. ; Reis, N.M.; Jones, C. I. ; Edwards, A. D.  Looping Flexible Fluoropolymer Microcapillary Film Extends Analysis Times for Vertical Microfluidic Blood Testing. Sensors 2024, 24, 5870. https://doi.org/10.3390/s24185870

2023

• Sariyer, R. M., Gill, K., Needs, S. H. , Hodge, D., Reis, N. M., Jones, C. I.  and Edwards, A. D.  (2023) Time- and distance-resolved robotic imaging of fluid flow in vertical microfluidic strips: a new technique for quantitative, multiparameter measurement of global haemostasis. Sensors and Diagnostics, 2 (6). pp. 1623-1637. ISSN 2635-0998 doi: https://doi.org/10.1039/D3SD00162H
• Sariyer, R. M., Edwards, A. D.  and Needs, S. H.  (2023) Open hardware for microfluidics: exploiting Raspberry Pi singleboard computer and camera systems for customisable laboratory instrumentation. Biosensors, 13 (10). 948. ISSN 2079-6374 doi: https://doi.org/10.3390/bios13100948
• Long, M. M. , Needs, S. H.  and Edwards, A. D.  (2023) Dilution reduces sample matrix effects for rapid, direct, and miniaturised phenotypic antibiotic susceptibility tests for bovine mastitis. Antibiotics, 12 (9). 1363. ISSN 2079-6382 doi: https://doi.org/10.3390/antibiotics12091363
• Needs, S. H. , Pivetal, J., Hayward, J., Kidd, S. P., Lam, H., Diep, T., Gill, K., Woodward, M., Reis, N. M. and Edwards, A. D.  (2023) Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and usability for detection of urinary tract infections in human urine. Sensors and Diagnostics, 2 (3). ISSN 2635-0998 doi: https://doi.org/10.1039/d2sd00138a
• Sarıyer, R. M. , Gill, K., Needs, S. H. , Hodge, D., Reis, N. M., Jones, C. I.  and Edwards, A. D.  (2023) Robotic microfluidic imaging of blood stimulation- towards high-throughput portable measurement of haemostasis. British Journal of Pharmacy, 8 (2). ISSN 2058-8356 doi: https://doi.org/10.5920/bjpharm.1365

2022

• Diep, T. T., Needs, S. H. , Bizley, S. and Edwards, A. D.  (2022) Rapid bacterial motility monitoring using inexpensive 3D-printed openflexure microscopy allows microfluidic antibiotic susceptibility testing. Micromachines, 13 (11). 1974. ISSN 2072-666X doi: https://doi.org/10.3390/mi13111974
• Long, M. M., Diep, T. T., Needs, S. H. , Ross, M. J. and Edwards, A. D.  (2022) PiRamid: A compact Raspberry Pi imaging box to automate small-scale time-lapse digital analysis, suitable for laboratory and field use. HardwareX, 12. e00377. ISSN 24680672 doi: https://doi.org/10.1016/j.ohx.2022.e00377
• Walker, S.  , Bravo, J. , Edwards, A.  (2022) User-friendly point-of-use instructions for home use diagnostic tests. University of Reading | doi: https://dx.doi.org/10.17864/1947.000419
• Walker, S.  , Bravo, J. , Edwards, A.  , Hart, J. , Little, G. (2022) Instructions for COVID-19 self-tests: what parts of the test are the most difficult to get right and how can information design help?. Information Design Journal ISSN: 1569-979X | doi: https://dx.doi.org/10.1075/idj.22011.wal

• Donmez, S. , Needs, S. , Osborn, H. , Reis, N. , Edwards, A.  (2022) Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques. Lab on a Chip , 22 (15). pp. 2820-2831. ISSN: 1473-0197 | doi: https://dx.doi.org/10.1039/d2lc00280a

• Diep, T.  , Bizley, S.  , Edwards, A.  (2022) 3D-printed dip slides miniaturize bacterial identification and antibiotic susceptibility tests allowing direct mastitis sample analysis. Micromachines , 13 (6). pp. 941. ISSN: 2072-666X | doi: https://dx.doi.org/10.3390/mi13060941
• Needs, S. , Saiprom, N. , Rafaque, Z. , Imtiaz, W. , Chantratita, N. , Runcharoen, C. , Thammachote, J. , Anun, S. , Peacock, S. , Ray, P.  , Andrews, S. , Edwards, A.  (2022) Miniaturised broth microdilution for simplified antibiotic susceptibility testing of Gram negative clinical isolates using microcapillary devices. Analyst ISSN: 0003-2654

• Needs, S.  , Sirivisoot, S.  , Jegouic, S. , Prommool, T. , Luangaram, P. , Srisawat, C. , Sriraksa, K. , Limpitikul, W. , Mairiang, D.  , Malasit, P. , Avirutnan, P.  , Puttikhunt, C. , Edwards, A.  (2022) Smartphone multiplex microcapillary diagnostics using Cygnus: development and evaluation of rapid serotype-specific NS1 detection with dengue patient samples. PLOS Neglected Tropical Diseases , 16 (4). pp 0. ISSN: 1935-2735 | doi: https://dx.doi.org/10.1371/journal.pntd.0010266

• Diep, T.  , Ray, P.  , Edwards, A.  (2022) Methods for rapid prototyping novel labware: using CAD and desktop 3D printing in the microbiology laboratory. Letters in Applied Microbiology , 74 (2). pp. 247-257. ISSN: 1472-765X | doi: https://dx.doi.org/10.1111/lam.13615

2021

• Reis, N. M. , Needs, S. H. , Jegouic, S. M., Gill, K. K., Sirivisoot, S., Howard, S., Kempe, J., Bola, S., Al-Hakeem, K., Jones, I. M. , Prommool, T., Luangaram, P., Avirutnan, P., Puttikhunt, C. and Edwards, A. D.  (2021) Gravity-driven microfluidic siphons: fluidic characterization and application to quantitative immunoassays. ACS Sensors, 6 (12). pp. 4338-4348. ISSN 2379-3694 doi: https://doi.org/10.1021/acssensors.1c01524
• Dobia, A. , Ryan, K. , BaHammam, A. , Abutaleb, M. , Alwadani, R. , Edwards, A.  (2021) The use of hypnotics for Insomnia Disorder in Saudi Arabia: a survey of patients’ experiences and perceptions. Sleep and Vigilance , 5 (2). pp. 235-244. ISSN: 2510-2265 | doi: https://dx.doi.org/10.1007/s41782-021-00151-7
• Diep, T. , Bizley, S. , Ray, P.  , Edwards, A.  (2021) MicroMI: a portable microbiological mobile incubator that uses inexpensive lithium power banks for field microbiology. HardwareX , 10 ISSN: 24680672 | doi: https://dx.doi.org/10.1016/j.ohx.2021.e00242
• Needs, S. , Donmez, S. , Edwards, A.  (2021) Direct microfluidic antibiotic resistance testing in urine with smartphone capture: significant variation in sample matrix interference between individual human urine samples. RSC Advances , 11 (60). pp. 38258-38263. ISSN: 2046-2069 | doi: https://dx.doi.org/10.1039/D1RA06867A
• Barbosa, A. , Edwards, A.  , Reis, N. (2021) Antibody surface coverage drives matrix interference in microfluidic capillary immunoassays. ACS Sensors ISSN: 2379-3694 | doi: https://dx.doi.org/10.1021/acssensors.1c00704
• Jegouic, S. M., Jones, I. M.  and Edwards, A. D.  (2021) Affordable mobile microfluidic diagnostics: minimum requirements for smartphones and digital imaging for colorimetric and fluorometric anti-dengue and anti-SARS-CoV-2 antibody detection. Wellcome Open Research, 6. 57. ISSN 2398-502X doi: https://doi.org/10.12688/wellcomeopenres.16628.1
• Needs, S., Osborn, H. and Edwards, A.  (2021) Counting bacteria in microfluidic devices: smartphone compatible ‘dip-and-test’ viable cell quantitation using resazurin amplified detection in microliter capillary arrays. Journal of Microbiological Methods. ISSN 0167-7012 (In Press)

2020

• Dönmez, S. İ., Needs, S. H. , Osborn, H. M. I. and Edwards, A. D.  (2020) Label-free smartphone quantitation of bacteria by darkfield imaging of light scattering in fluoropolymer micro capillary film allows portable detection of bacteriophage lysis. Sensors and Actuators B: Chemical, 323. p. 128645. ISSN 0925-4005 doi: https://doi.org/10.1016/j.snb.2020.128645
• Needs, S., Donmez, S. I., Bull, S., McQuaid, C., Osborn, H. M. I. and Edwards, A.  (2020) Challenges in microfluidic and point-of-care phenotypic antimicrobial resistance tests. Frontiers in Mechanical Engineering, 6. ISSN 2297-3079 doi: https://doi.org/10.3389/fmech.2020.00073
• Bull, S., Needs, S. and Edwards, A., (2020) Building open lab hardware to tackle antimicrobial resistance. Open Research Case Studies. Report. University of Reading doi: https://doi.org/10.17864/1926.89600
• Needs SH, Bull SP, Bravo J et al. Remote videolink observation of model home sampling and home testing devices to simplify usability studies for point-of-care diagnostics [version 1; peer review: awaiting peer review]. Wellcome Open Res 2020, 5:174 (https://doi.org/10.12688/wellcomeopenres.16105.1)

2019

• Dobia, A., Ryan, K., BaHammam, A. and Edwards, A. (2019) Using benzodiazepines and Z-Drugs for managing primary insomnia in adults in Saudi Arabia: an e-Delphi study to aid the development of clinical guidelines. Sleep and Breathing, 23 (4). pp. 1133-1139. ISSN 1520-9512 doi: https://doi.org/10.1007/s11325-019-01794-7
• Needs SH, Diep TT, Bull SP, Lindley-Decaire A, Ray P, Edwards AD (2019) Exploiting open source 3D printer architecture for laboratory robotics to automate high-throughput time-lapse imaging for analytical microbiology. PLoS ONE 14(11): e0224878. https://doi.org/10.1371/journal.pone.022487
• Dobia, A., Ryan, K., BaHammam, A. and Edwards, A. (2019) Using benzodiazepines and Z-Drugs for managing primary insomnia in adults in Saudi Arabia: an e-Delphi study to aid the development of clinical guidelines. Sleep and Breathing. ISSN 1520-9512 doi: https://doi.org/10.1007/s11325-019-01794-7 (In Press)

2018

• Gadd, A. J. R., Castelletto, V., Kabova, E., Shankland, K., Perrie, Y., Hamley, I., Cobb, A. J. A., Greco, F. and Edwards, A. D. (2018) High potency of lipid conjugated TLR7 agonist requires nanoparticulate or liposomal formulation. European Journal of Pharmaceutical Sciences, 123. pp. 268-276. ISSN 0928-0987 doi: https://doi.org/10.1016/j.ejps.2018.07.048
• Thangsupanimitchai, N. and Edwards, A. D. (2018) Mixing is required for uniform reconstitution of filter-dried protein antigens in a single-injection vaccine formulation.Vaccine, 36 (3). pp. 5058-5064. ISSN 0264-410X doi: https://doi.org/10.1016/j.vaccine.2018.07.001
• Dahal, L. N., Gadd, A., Edwards, A. D., Cragg, M. S. and Beers, S. A. (2018) UC-1V150, a potent TLR7 agonist capable of activating macrophages and potentiating mAb-mediated target cell deletion. Scandinavian Journal of Immunology, 87 (6). e12666. ISSN 0300-9475 doi: https://doi.org/10.1111/sji.12666

2017

• Pivetal, J., Pereira, F. M., Barbosa, A. I., Castanheira, A. P., Reis, N. M. and Edwards, A. D. (2017)Covalent immobilisation of antibodies in Teflon-FEP microfluidic devices for sensitive quantification of clinically relevant protein biomarkers. The Analyst, 142 (6). pp. 959-968. ISSN 0003-2654 doi: https://doi.org/10.1039/C6AN02622B

2016

• Reis, N. M., Pivetal, J., Loo-Zazueta, A. L., Barros, J. M. S. and Edwards, A. D. (2016) Lab on a stick: multi-analyte cellular assays in a microfluidic dipstick. Lab on a Chip, 16 (15). pp. 2891-2899. ISSN 1473-0189 doi: https://doi.org/10.1039/C6LC00332J
• de Barros, J. M.S., Costabile, A., Charalampopoulos, D., Khutoryanskiy, V. V. and Edwards, A. D.(2016) Evaluating and optimizing oral formulations of live bacterial vaccines using a gastro-small intestine model. European Journal of Pharmaceutics and Biopharmaceutics, 102. pp. 115-122. ISSN 0939-6411 doi: https://doi.org/10.1016/j.ejpb.2016.03.010
• Loughborough University (2016) Capillary assay device with internal hydrophilic coating.WO2016012778. doi: WO2016012778

2015

• de Barros, J. M.S., Lechner, T., Charalampopoulos, D., Khutoryanskiy, V. V. and Edwards, A. D.(2015) Enteric coated spheres produced by extrusion/spheronization provide effective gastric protection and efficient release of live therapeutic bacteria. International Journal of Pharmaceutics, 493 (1-2). pp. 483-494. ISSN 0378-5173 doi: https://doi.org/10.1016/j.ijpharm.2015.06.051
• Barbosa, A. I., Gehlot, P., Sidapra, K., Edwards, A. D. and Reis, N. M. (2015) Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70. pp. 5-14. ISSN 0956-5663 doi: https://doi.org/10.1016/j.bios.2015.03.006
• Castanheira, A. P., Barbosa, A. I., Edwards, A. D. and Reis, N. M. (2015) Multiplexed femtomolar quantitation of human cytokines in a fluoropolymer microcapillary film. Analyst, 140 (16). pp. 5609-5618. ISSN 0003-2654 doi: https://doi.org/10.1039/C5AN00238A
• Gadd, A. J. R., Greco, F., Cobb, A. J. A. and Edwards, A. D. (2015) Targeted activation of toll-like receptors: conjugation of a toll-like receptor 7 agonist to a monoclonal antibody maintains antigen binding and specificity. Bioconjugate Chemistry, 26 (8). pp. 1743-1752. ISSN 1043-1802 doi: https://doi.org/10.1021/acs.bioconjchem.5b00302

2014

• de Barros, J. M. S., Scherer, T., Charalampopoulos, D., Khutoryanskiy, V. V. and Edwards, A. D.(2014) A laminated polymer film formulation for enteric delivery of live vaccine and probiotic bacteria. Journal of Pharmaceutical Sciences, 103 (7). pp. 2022-2032. ISSN 1520-6017 doi: https://doi.org/10.1002/jps.23997
• Mahbubani, K. T., Slater, N. K. H. and Edwards, A. D. (2014) Protection of dried probiotic bacteria from bile using bile adsorbent resins. New Biotechnology, 31 (1). pp. 69-72. ISSN 1871-6784 doi: https://doi.org/10.1016/j.nbt.2013.09.001
• Barbosa, A. I., Castanheira, A. P., Edwards, A. D. and Reis, N. M. (2014) A Lab-in-a-briefcase for rapid prostate specific antigen (PSA) screening from whole blood. Lab on a Chip, 14 (16). pp. 2918-2928. ISSN 1473-0197 doi: https://doi.org/10.1039/C4LC00464G

2013

• Cook, M. T., Saratoon, T., Tzortzis, G., Edwards, A., Charalampopoulos, D. and Khutoryanskiy, V. V. (2013) CLSM method for the dynamic observation of pH change within polymer matrices for oral delivery. Biomacromolecules, 14 (2). pp. 387-393. ISSN 1525-7797 doi: https://doi.org/10.1021/bm301569r
• Edwards, A.D. (2013) What have dendritic cells ever done for adjuvant design? Cellular and molecular methods for the rational development of vaccine adjuvants. In: Flower , D. R. and Perrie, Y. (eds.) Immunomic Discovery of Adjuvants and Candidate Subunit Vaccines. Immunomics reviews (5). Springer, New York , pp. 131-154. ISBN 978-1-4614-5070-2 doi: https://doi.org/10.1007/978-1-4614-5070-2_8

2012

• Jopeace, A.L., Howard, C.B., Murton, B.L., Edwards, A. and Monie, T.P. (2012) Pattern recognition receptors and infectious diseases. In: Insight and control of infectious disease in global scenario. InTech Open. ISBN 9789535103196
• Gerrard, S. E., Baniecki, M. L., Sokal, D. C., Morris, M. K., Urdaneta-Hartmann, S., Krebs, F. C., Wigdahl, B., Abrams, B. F., Hanson, C. V., Slater, N. K.H. and Edwards, A. D. (2012) A nipple shield delivery system for oral drug delivery to breastfeeding infants : Microbicide delivery to inactivate HIV. International Journal of Pharmaceutics, 434 (1-2). 224- 234. ISSN 0378-5173 doi: https://doi.org/10.1016/j.ijpharm.2012.05.035

2011

• Edwards, A. D., Reis, N. M., Slater, N. K. H. and Mackley, M. R. (2011) A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. Lab on a Chip, 11 (24). pp. 4267-4273. ISSN 1473-0197 doi: https://doi.org/10.1039/C0LC00357C
• Cambridge Enterprise Ltd (2011) Immunoassays, methods for carrying out immunoassays, immunoassay kits and method for manufacturing immunoassay kits. WO/2011/117579. doi: WO/2011/117579

2010

• Edwards, A. D., Chatterjee, P., Mahbubani, K. T., Reis, C. M. and Slater, N. K.H. (2010) Optimal protection of stabilised dry live bacteria from bile toxicity in oral dosage forms by bile acid adsorbent resins. Chemical Engineering Science, 65 (16). pp. 4844-4854. ISSN 0009-2509 doi: https://doi.org/10.1016/j.ces.2010.05.030
• Cambridge Enterprise Ltd (2010) Formulations of viable cells for oral delivery.WO2010079343. doi: WO2010079343

2009

• Cheeks, M. C., Edwards, A. D., Arnot, C. J. and Slater, N. K.H. (2009) Gene transfection of HEK cells on supermacroporous polyacrylamide monoliths: a comparison of transient and stable recombinant protein expression in perfusion culture. New Biotechnology, 26 (6). pp. 289-299. ISSN 1871-6784 doi: https://doi.org/10.1016/j.nbt.2009.08.006
• Edwards, A. D. and Slater, N. K.H. (2009) Protection of live bacteria from bile acid toxicity using bile acid adsorbing resins. Vaccine, 27 (29). pp. 3897-3903. ISSN 0264-410X doi: https://doi.org/10.1016/j.vaccine.2009.04.006

2008

• Edwards, A. D. and Slater, N. K.H. (2008) Formulation of a live bacterial vaccine for stable room temperature storage results in loss of acid, bile and bile salt resistance. Vaccine, 26 (45). pp. 5675-5678. ISSN 0264-410X doi: https://doi.org/10.1016/j.vaccine.2008.08.024

2006

• Carr, J. M., Carrasco, M. J., Thaventhiran, J. E. D., Bambrough, P. J., Kraman, M., Edwards, A. D., Al-Shamkhani, A. and Fearon, D. T. (2006) CD27 mediates interleukin-2-independent clonal expansion of the CD8+ T cell without effector differentiation. Proceedings of the National Academy of Sciences, 103 (51). p. 19454. ISSN 0027-8424 doi: https://doi.org/10.1073/pnas.0609706104

2005

• Rogers, N. C., Slack, E. C., Edwards, A. D., Nolte, M. A., Schulz, O., Schweighoffer, E., Williams, D. L., Gordon, S., Tybulewicz, V. L. and Brown, G. D. (2005) Syk-Dependent Cytokine Induction by Dectin-1 Reveals a Novel Pattern Recognition Pathway for C Type Lectins. Immunity, 22 (4). p. 507. ISSN 10747613 doi: https://doi.org/10.1016/j.immuni.2005.03.004

2003

• Edwards, A. D., Chaussabel, D., Tomlinson, S., Schulz, O., Sher, A. and Reis e Sousa, C. (2003)Relationships Among Murine CD11chigh Dendritic Cell Subsets as Revealed by Baseline Gene Expression Patterns. The Journal of Immunology, 171 (1). pp. 47-60. ISSN 0022-1767 doi: https://doi.org/10.4049/jimmunol.171.1.47
• Edwards, A. D., Diebold, S. S., Slack, E. M. C., Tomizawa, H., Hemmi, H., Kaisho, T., Akira, S. and Sousa, C. R. e. (2003) Toll-like receptor expression in murine DC subsets: lack of TLR7 expression by CD8α+ DC correlates with unresponsiveness to imidazoquinolines. European Journal of Immunology, 33 (4). p. 827. ISSN 00142980 doi: https://doi.org/10.1002/eji.200323797

2002

• Edwards, A. D., Manickasingham, S. P., Sporri, R., Diebold, S. S., Schulz, O., Sher, A., Kaisho, T., Akira, S. and Reis e Sousa, C. (2002) Microbial Recognition Via Toll-Like Receptor-Dependent and -Independent Pathways Determines the Cytokine Response of Murine Dendritic Cell Subsets to CD40 Triggering. The Journal of Immunology, 169 (7). pp. 3652-3660. ISSN 0022-1767 doi: https://doi.org/10.4049/jimmunol.169.7.3652

2000

• Schulz, O., Edwards, A. D., Schito, M., Aliberti, J., Manickasingham, S., Sher, A. and Reis e Sousa, C. (2000) CD40 Triggering of Heterodimeric IL-12 p70 Production by Dendritic Cells In Vivo Requires a Microbial Priming Signal. Immunity, 13 (4). p. 453. ISSN 10747613 doi: https://doi.org/10.1016/S1074-7613(00)00045-5