Experiment Title: “Symbiotic nitrogen fixation reduces belowground biomass carbon costs of nitrogen acquisition under low, but not high, nitrogen availability” Institutions responsible:

 

Experiment Description: The purpose of this experiment was to understand how nitrogen acquisition strategy, namely the ability to acquire nitrogen through symbiotic nitrogen fixation, influenced carbon costs to acquire nitrogen. The experiment quantified carbon costs to acquire nitrogen in Glycine max (soybean) seedlings that received one of two inoculation treatments (one without inoculation, one with inoculation of Bradyrhizobium japonicum) and one of two nitrogen fertilization treatments (70 ppm N, 630 ppm N) in a full-factorial greenhouse experiment.
Variables being tested: Experimental Details (factorial design, replication, lab/field/ controls, vegetation type): Data availability: Study limitations:
  • Carbon costs to acquire nitrogen (the ratio of belowground carbon allocation to whole-plant nitrogen biomass)
  • Root nodule biomass
  • Root nodule biomass: root biomass
  • Total leaf area
  • Total biomass
Greenhouse experiment where soybean seedlings received one of two inoculation treatments (one without inoculation, one with inoculation of Bradyrhizobium japonicum) and one of two nitrogen fertilization treatments (70 ppm N, 630 ppm N) in a full-factorial greenhouse experiment. Individuals were harvested after six weeks of development. Data are available on Zenodo: here

Paper is currently available at AoB Plants: here

 

Carbon costs to acquire nitrogen do not include carbon allocated belowground through root exudates or lost through root exudation