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Research Seminar: Gene Synthesis
Latest Developments in Synthetic Biology : Gene Synthesis | Protein Expression | CRISPR
Talk by Gavin Allsop (Fisher Scientific)
Gene synthesis has become the most cost effective way to increase protein quality and yield, but more interestingly affords us the ability to develop vastly improved protein expression platforms and genome editing tools.
Our proprietary algorithm GeneOptimizer has shown vast improvements to protein expression, especially when used alongside ExpiCHO, Expi293 and ExpiSf. Here, one should come to expect expression yields in excess of 100s mg/L. Furthermore, with improvements to sequence clonability, site saturation mutagenesis and TRIM technology, the R&D team from Thermo Fisher Scientific have come to expect editing efficiencies of 90% in primary T cells; through updated Cas9 RNP based editing strategies.
This seminar promises to explore peer reviewed data leading up to these developments. To look at improvements to HDR template design, editing in PAM deserts, functional protein screening and offers and end to off the shelf vector limitations.